In vitrocharacterization of human dental pulp stem cells isolated by three different methods
نویسندگان
چکیده
منابع مشابه
In vitro characterization of human dental pulp stem cells isolated by three different methods
OBJECTIVES In this study, we characterized human dental pulp cells (HDPCs) obtained by different culture methods to establish the most suitable methodology for dental tissue engineering and regenerative endodontic applications. MATERIALS AND METHODS HDPCs were isolated by the outgrowth method (HDPCs-OG), the enzymatic digestion method (collagenase/dispase/trypsin, HDPCs-ED), or the combinatio...
متن کاملDifferent isolation methods of dental pulp stem cells
Considering the ease of isolation and high expansion potential of pulp stem/progenitor cells isolated from wisdom and primary teeth they have been implicated as the most reliable autologous cell source in dental tissue engineering. Meanwhile, different isolation methods have remarkable impacts on the expansion potential of adult stem cells. In enzymatic digestion method extracted teeth as denta...
متن کاملTransdifferentiation of Human Dental Pulp Stem Cells Into Oligoprogenitor Cells
Introduction: The nerve fibers in central nervous system are surrounded by myelin sheet which is formed by oligodendrocytes. Cell therapy based on oligodendrocytes and their precursors transplantation can hold a promising alternative treatment for myelin sheet repair in demyelinating diseases. Methods: Human Dental Pulp Stem Cells (hDPSCs) are noninvasive, autologous and easy available s...
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Objective(s): Biocompatibility of dental biomaterials plays a critical role in regeneration of dental stem cells. The aim of present study was to evaluate the effects of novel biomaterials of TheraCal-LC (TheraCal; Bisco), Angelus mineral trioxide aggregate (MTA; Angelus), calcium-enriched mixture (CEM; BioniqueDent), and Biodentine (Septodont) on viability of human de...
متن کاملHuman dental pulp stem cells derived from different cryopreservation methods of human dental pulp tissues of diseased teeth.
BACKGROUND Successful isolation of human dental pulp stem cells (hDPSCs) has been documented at least 120h after tooth extraction. Viable hDPSCs have been isolated chiefly from cryopreserved healthy molar teeth and their undigested dental pulp tissue. Isolation of hDPSCs from diseased but vital teeth after cryopreservation has not been reported. This study aimed to isolate hDPSCs from cryoprese...
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ژورنال
عنوان ژورنال: Restorative Dentistry & Endodontics
سال: 2016
ISSN: 2234-7658,2234-7666
DOI: 10.5395/rde.2016.41.4.283